For a Reliable, Easy & Convenient High Quality DNA Isolation from Fresh or Frozen Stool Samples
HigherPurity™ Stool DNA Isolation Kit provides a reliable, easy and convenient technique to isolate high quality DNA from fresh or frozen stool samples. The kit uses HigherPurity™ breakthrough technology based in DNA ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate.
Fecal samples are rapidly and efficiently lysed. The sample DNA is then bound to the surface of a Spin Filter membrane and washed and the bound DNA is then desorbed from the surface of the Spin Filter column. The inhibitors of the downstream PCR will be removed by utilizing the DNA binding column and the buffers system included in the kit.
Compatible with DNA stool preservation solutions, such as the Stool DNA Collection & Stabilization Kit.
Advantages & Features:
- High purity: eliminates humic acid, polysaccharides, phenol compounds and enzyme inhibitors from stool samples.
- Great performance: expected yields are 10 ~25 µg.
- Time-saving protocol: rapid isolation of ready-to-use DNA within 54 minutes with minimal handling steps.
- Safe and convenient: avoids phenol/chloroform extraction.
- Sample Size: up to 200 mg of fresh or frozen stool sample.
- High Binding capacity: up to 60 µg DNA per column.
- High quality DNA (A260/280= 1.8 ± 0.2; A260/230= 1.8 ± 0.2)
- Extremely easy procedure: to isolate high quality DNA from fresh or frozen stool samples.
*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.
Research or further manufacturing use only, not for food or drug use.